Pelkmanslab: Image-based transcriptomics

High-content single-molecule fluorescence in situ hybridization to measure transcript copy number and subcellular localization in thousands of single cells.

Control of Transcript Variability

If you like heterogeneity of cells as much as we do, you might want to return soon to this page. In the meantime, we hope that you equally enjoy browsing the single-cell distributions of endogenous transcripts at single-molecule resolution.

Browse data

Computer Vision

Here we describe in detail the algorithms to extract, at high-throughput, robust multivariate feature sets of transcript molecule abundance, localization and patterning.

Read the paper Get the code

Example data

Image-based transcriptomics

Here we report image-based transcriptomics in thousands of single human cells at single-molecule resolution and possible applications of this method.

Read the paper News & Views